Mgme1 (NM_028984) Mouse Recombinant Protein

• Background:

  Metal-dependent single-stranded DNA (ssDNA) exonuclease involved in mitochondrial genome maintenance. Has preference for 5'-3' exonuclease activity but is also capable of endoduclease activity on linear substrates. Necessary for maintenance of proper 7S DNA levels. Probably involved in mitochondrial DNA (mtDNA) repair, possibly via the processing of displaced DNA containing Okazaki fragments during RNA-primed DNA synthesis on the lagging strand or via processing of DNA flaps during long-patch base excision repair (By similarity) . Specifically binds 5-hydroxymethylcytosine (5hmC) -containing DNA in stem cells.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  8430406I07Rik; AI426476

• Gene ID:

  74528

• UniProt:

  Q9CXC3

• Accession Number mRNA:

  NM_028984

• Chromosomal Location:

  2 G1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  38.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Mgme1

• Species:

  Mouse

• Protein ID:

  NP_083260

• Overview:

  Purified recombinant protein of Mouse mitochondrial genome maintenance exonuclease 1 (Mgme1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/74528

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9CXC3

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_028984

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_083260