Trp53 (NM_011640) Mouse Recombinant Protein

• Background:

  This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined. [provided by RefSeq, Jul 2008]

• Synonyms:

  Bbl; bfy; bhy; p4; p5; p44; p53; Tp53

• Gene ID:

  22059

• UniProt:

  P02340, Q549C9, Q3UGQ1

• Accession Number mRNA:

  NM_011640

• Chromosomal Location:

  11 42.83 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  43.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Trp53

• Species:

  Mouse

• Protein ID:

  NP_035770

• Overview:

  Purified recombinant protein of Mouse transformation related protein 53 (Trp53), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/22059

• Uniprot URL:

  https://www.uniprot.org/uniprot/P02340

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_011640

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_035770