Ido1 (NM_008324) Mouse Recombinant Protein

• Background:

  Catalyzes the first and rate limiting step of the catabolism of the essential amino acid tryptophan along the kynurenine pathway. Involved in the peripheral immune tolerance, contributing to maintain homeostasis by preventing autoimmunity or immunopathology that would result from uncontrolled and overreacting immune responses. Tryptophan shortage inhibits T lymphocytes division and accumulation of tryptophan catabolites induces T-cell apoptosis and differentiation of regulatory T-cells. Acts as a suppressor of anti-tumor immunity (PubMed:25691885) . Limits the growth of intracellular pathogens by depriving tryptophan. Protects the fetus from maternal immune rejection (Ref. 3) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Ido; Indo

• Gene ID:

  15930

• UniProt:

  P28776

• Accession Number mRNA:

  NM_008324

• Chromosomal Location:

  8 A2

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  45.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ido1

• Species:

  Mouse

• Protein ID:

  NP_032350

• Overview:

  Purified recombinant protein of Mouse indoleamine 2, 3-dioxygenase 1 (Ido1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/15930

• Uniprot URL:

  https://www.uniprot.org/uniprot/P28776

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008324

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032350