Eif4a2 (NM_013506) Mouse Recombinant Protein

• Background:

  ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  4833432N07Rik; BM-010; Ddx2b; eIF-4A-II; Eif4; eIF4A-II

• Gene ID:

  13682

• UniProt:

  P10630, Q52KC1

• Accession Number mRNA:

  NM_013506

• Chromosomal Location:

  16 13.93 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  46.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Eif4a2

• Species:

  Mouse

• Protein ID:

  NP_038534

• Overview:

  Purified recombinant protein of Mouse eukaryotic translation initiation factor 4A2 (Eif4a2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/13682

• Uniprot URL:

  https://www.uniprot.org/uniprot/P10630

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_013506

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_038534