Pgk1 (NM_008828) Mouse Recombinant Protein

• Background:

  The protein encoded by this gene is a glycolytic enzyme that catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate. Additionally, this protein is secreted by tumor cells where it participates in angiogenesis by functioning to reduce disulfide bonds in the serine protease, plasmin, which consequently leads to the release of the tumor blood vessel inhibitor angiostatin. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions. Many pseudogenes of this gene are found throughout the mouse genome. [provided by RefSeq, Jan 2014]

• Synonyms:

  Pgk-; Pgk-1

• Gene ID:

  18655

• UniProt:

  P09411

• Accession Number mRNA:

  NM_008828

• Chromosomal Location:

  X 47.36 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  44.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Pgk1

• Species:

  Mouse

• Protein ID:

  NP_032854

• Overview:

  Purified recombinant protein of Mouse phosphoglycerate kinase 1 (Pgk1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/18655

• Uniprot URL:

  https://www.uniprot.org/uniprot/P09411

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008828

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032854