Fbxl2 (NM_178624) Mouse Recombinant Protein

• Background:

  Calcium-activated substrate recognition component of the SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex, SCF (FBXL2), which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 does not seem to target phosphodegron within its substrates but rather calmodulin-binding motifs and is thereby antagonized by calmodulin. This is the case for the cyclins CCND2 and CCND3 which polyubiquitination and subsequent degradation are inhibited by calmodulin. Through CCND2 and CCND3 degradation induces cell-cycle arrest in G (0) . SCF (FBXL2) also mediates PIK3R2 ubiquitination and proteasomal degradation thereby regulating phosphatidylinositol 3-kinase signaling and autophagy (By similarity) . PCYT1A monoubiquitination by SCF (FBXL2) and subsequent degradation regulates synthesis of phosphatidylcholine, which is utilized for formation of membranes and of pulmonary surfactant (PubMed:21343341) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2810423A21Rik; Fbl3

• Gene ID:

  72179

• UniProt:

  Q8BH16, Q4VA21

• Accession Number mRNA:

  NM_178624

• Chromosomal Location:

  9 F3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  46.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Fbxl2

• Species:

  Mouse

• Protein ID:

  NP_848739

• Overview:

  Purified recombinant protein of Mouse F-box and leucine-rich repeat protein 2 (Fbxl2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/72179

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8BH16

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_178624

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_848739