Entpd5 (NM_001026214) Mouse Recombinant Protein

• Background:

  Uridine diphosphatase (UDPase) that promotes protein N-glycosylation and ATP level regulation. UDP hydrolysis promotes protein N-glycosylation and folding in the endoplasmic reticulum, as well as elevated ATP consumption in the cytosol via an ATP hydrolysis cycle. Together with CMPK1 and AK1, constitutes an ATP hydrolysis cycle that converts ATP to AMP and results in a compensatory increase in aerobic glycolysis. The nucleotide hydrolyzing preference is GDP > IDP > UDP, but not any other nucleoside di-, mono- or triphosphates, nor thiamine pyrophosphate. Plays a key role in the AKT1-PTEN signaling pathway by promoting glycolysis in proliferating cells in response to phosphoinositide 3-kinase (PI3K) signaling.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI196558; AI987697; Cd39l4; ER-UDPase; mNTPase; NTPDase-5; NTPDase5; Pcph

• Gene ID:

  12499

• UniProt:

  Q9WUZ9

• Accession Number mRNA:

  NM_001026214

• Chromosomal Location:

  12 39.18 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  47.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Entpd5

• Species:

  Mouse

• Protein ID:

  NP_001021385

• Overview:

  Purified recombinant protein of Mouse ectonucleoside triphosphate diphosphohydrolase 5 (Entpd5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/12499

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9WUZ9

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001026214

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001021385