Pdk1 (NM_172665) Mouse Recombinant Protein

• Background:

  Kinase that plays a key role in regulation of glucose and fatty acid metabolism and homeostasis via phosphorylation of the pyruvate dehydrogenase subunits PDHA1 and PDHA2. This inhibits pyruvate dehydrogenase activity, and thereby regulates metabolite flux through the tricarboxylic acid cycle, down-regulates aerobic respiration and inhibits the formation of acetyl-coenzyme A from pyruvate. Plays an important role in cellular responses to hypoxia and is important for cell proliferation under hypoxia. Protects cells against apoptosis in response to hypoxia and oxidative stress (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  B830012B01; D530020C15Rik

• Gene ID:

  228026

• UniProt:

  Q8BFP9

• Accession Number mRNA:

  NM_172665

• Chromosomal Location:

  2 C3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  48.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Pdk1

• Species:

  Mouse

• Protein ID:

  NP_766253

• Overview:

  Purified recombinant protein of Mouse pyruvate dehydrogenase kinase, isoenzyme 1 (Pdk1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/228026

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8BFP9

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_172665

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_766253