Ifit2 (NM_008332) Mouse Recombinant Protein

• Background:

  IFN-induced antiviral protein which inhibits expression of viral messenger RNAs lacking 2'-O-methylation of the 5' cap. The ribose 2'-O-methylation would provide a molecular signature to distinguish between self and non-self mRNAs by the host during viral infection. Viruses evolved several ways to evade this restriction system such as encoding their own 2'-O-methylase for their mRNAs or by stealing host cap containing the 2'-O-methylation (cap snatching mechanism) . Binds AU-rich viral RNAs, with or without 5' triphosphorylation, RNA-binding is required for antiviral activity. Can promote apoptosis.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AV302338; Ifi54; P54

• Gene ID:

  15958

• UniProt:

  Q64112, Q6GTM0

• Accession Number mRNA:

  NM_008332

• Chromosomal Location:

  19 C1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  55.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ifit2

• Species:

  Mouse

• Protein ID:

  NP_032358

• Overview:

  Purified recombinant protein of Mouse interferon-induced protein with tetratricopeptide repeats 2 (Ifit2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/15958

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q64112

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008332

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032358