Sptlc1 (NM_009269) Mouse Recombinant Protein

• Background:

  Serine palmitoyltransferase (SPT) (PubMed:28100772) . The heterodimer formed with SPTLC2 or SPTLC3 constitutes the catalytic core. The composition of the serine palmitoyltransferase (SPT) complex determines the substrate preference. The SPTLC1-SPTLC2-SPTSSA complex shows a strong preference for C16-CoA substrate, while the SPTLC1-SPTLC3-SPTSSA isozyme uses both C14-CoA and C16-CoA as substrates. The SPTLC1-SPTLC2-SPTSSB complex displays a strong preference for C18-CoA substrate, while the SPTLC1-SPTLC3-SPTSSB isozyme has the ability to use a broader range of acyl-CoAs (By similarity) . Required for adipocyte cell viability and metabolic homeostasis (PubMed:28100772) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AW552086; C77762; E030036H05; Lcb1

• Gene ID:

  268656

• UniProt:

  O35704

• Accession Number mRNA:

  NM_009269

• Chromosomal Location:

  13 B1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  53 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Sptlc1

• Species:

  Mouse

• Protein ID:

  NP_033295

• Overview:

  Purified recombinant protein of Mouse serine palmitoyltransferase, long chain base subunit 1 (Sptlc1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/268656

• Uniprot URL:

  https://www.uniprot.org/uniprot/O35704

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_009269

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_033295