Arih2 (NM_011790) Mouse Recombinant Protein

• Background:

  E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 UBE2L3 (By similarity) . Acts as an atypical E3 ubiquitin-protein ligase by working together with cullin-5-RING ubiquitin ligase complex (ECS complex, also named CRL5 complex) and initiating ubiquitination of ECS substrates: associates with ECS complex and specifically mediates addition of the first ubiquitin on ECS targets (By similarity) . The initial ubiquitin is then elongated (By similarity) . E3 ubiquitin-protein ligase activity is activated upon binding to neddylated form of the ECS complex. Mediates 'Lys-6', 'Lys-48'-and 'Lys-63'-linked polyubiquitination. May play a role in myelopoiesis (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI843547; ARI2; TRIAD1; UIP48

• Gene ID:

  23807

• UniProt:

  Q9Z1K6, Q3TK92

• Accession Number mRNA:

  NM_011790

• Chromosomal Location:

  9 F2

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  57.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Arih2

• Species:

  Mouse

• Protein ID:

  NP_035920

• Overview:

  Purified recombinant protein of Mouse ariadne RBR E3 ubiquitin protein ligase 2 (Arih2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/23807

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9Z1K6

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_011790

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_035920