Rbck1 (NM_001083921) Mouse Recombinant Protein

• Background:

  E3 ubiquitin-protein ligase, which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, such as UBE2L3/UBCM4, and then transfers it to substrates. Functions as an E3 ligase for oxidized IREB2 and both heme and oxygen are necessary for IREB2 ubiquitination. Promotes ubiquitination of TAB2 and IRF3 and their degradation by the proteasome. Component of the LUBAC complex which conjugates linear ('Met-1'-linked) polyubiquitin chains to substrates and plays a key role in NF-kappa-B activation and regulation of inflammation. LUBAC conjugates linear polyubiquitin to IKBKG and RIPK1 and is involved in activation of the canonical NF-kappa-B and the JNK signaling pathways. Linear ubiquitination mediated by the LUBAC complex interferes with TNF-induced cell death and thereby prevents inflammation. LUBAC is recruited to the TNF-R1 signaling complex (TNF-RSC) following polyubiquitination of TNF-RSC components by BIRC2 and/or BIRC3 and to conjugate linear polyubiquitin to IKBKG and possibly other components contributing to the stability of the complex. Together with OTULIN, the LUBAC complex regulates the canonical Wnt signaling during angiogenesis. Binds polyubiquitin of different linkage types (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AL033326; HOIL-1; HOIL-1L; Ubce7ip3; UIP28

• Gene ID:

  24105

• UniProt:

  Q9WUB0

• Accession Number mRNA:

  NM_001083921

• Chromosomal Location:

  2 G3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  56.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Rbck1

• Species:

  Mouse

• Protein ID:

  NP_001077390

• Overview:

  Purified recombinant protein of Mouse RanBP-type and C3HC4-type zinc finger containing 1 (Rbck1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/24105

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9WUB0

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001083921

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001077390