Wasl (NM_028459) Mouse Recombinant Protein

• Background:

  Regulates actin polymerization by stimulating the actin-nucleating activity of the Arp2/3 complex. Involved in various processes, such as mitosis and cytokinesis, via its role in the regulation of actin polymerization. Together with CDC42, involved in the extension and maintenance of the formation of thin, actin-rich surface projections called filopodia. In addition to its role in the cytoplasm, also plays a role in the nucleus by regulating gene transcription, probably by promoting nuclear actin polymerization (By similarity) . Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression (PubMed:12871950) . Plays a role in dendrite spine morphogenesis (PubMed:25851601) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2900021I12Rik; 3110031I02Rik; N-WASP

• Gene ID:

  73178

• UniProt:

  Q91YD9, Q3TXX8, Q6PDL3

• Accession Number mRNA:

  NM_028459

• Chromosomal Location:

  6 A3.1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  54.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Wasl

• Species:

  Mouse

• Protein ID:

  NP_082735

• Overview:

  Purified recombinant protein of Mouse Wiskott-Aldrich syndrome-like (human) (Wasl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/73178

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q91YD9

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_028459

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_082735