Podxl (NM_013723) Mouse Recombinant Protein

• Background:

  Involved in the regulation of both adhesion and cell morphology and cancer progression. Function as an anti-adhesive molecule that maintains an open filtration pathway between neighboring foot processes in the podocyte by charge repulsion. Acts as a pro-adhesive molecule, enhancing the adherence of cells to immobilized ligands, increasing the rate of migration and cell-cell contacts in an integrin-dependent manner. Induces the formation of apical actin-dependent microvilli. Involved in the formation of a preapical plasma membrane subdomain to set up initial epithelial polarization and the apical lumen formation during renal tubulogenesis. Plays a role in cancer development and aggressiveness by inducing cell migration and invasion through its interaction with the actin-binding protein EZR. Affects EZR-dependent signaling events, leading to increased activities of the MAPK and PI3K pathways in cancer cells.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AW121214; Ly102; PC; PCLP-1; Pclp1; Podxl1

• Gene ID:

  27205

• UniProt:

  Q9R0M4, Q791G4

• Accession Number mRNA:

  NM_013723

• Chromosomal Location:

  6 12.57 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  53.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Podxl

• Species:

  Mouse

• Protein ID:

  NP_038751

• Overview:

  Purified recombinant protein of Mouse podocalyxin-like (Podxl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/27205

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9R0M4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_013723

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_038751