Sirpa (NM_001177646) Mouse Recombinant Protein

• Background:

  Immunoglobulin-like cell surface receptor for CD47. Acts as docking protein and induces translocation of PTPN6, PTPN11 and other binding partners from the cytosol to the plasma membrane. Supports adhesion of cerebellar neurons, neurite outgrowth and glial cell attachment. May play a key role in intracellular signaling during synaptogenesis and in synaptic function. Involved in the negative regulation of receptor tyrosine kinase-coupled cellular responses induced by cell adhesion, growth factors or insulin. Mediates negative regulation of phagocytosis, mast cell activation and dendritic cell activation. CD47 binding prevents maturation of immature dendritic cells and inhibits cytokine production by mature dendritic cells (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI835480; Bit; CD172a; P84; Ptpns1; SHP-1; SHPS-1; SIRP

• Gene ID:

  19261

• UniProt:

  P97797

• Accession Number mRNA:

  NM_001177646

• Chromosomal Location:

  2 63.19 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  56.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Sirpa

• Species:

  Mouse

• Overview:

  Purified recombinant protein of Mouse signal-regulatory protein alpha (Sirpa), transcript variant 2, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/19261

• Uniprot URL:

  https://www.uniprot.org/uniprot/P97797

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001177646