Alpl (NM_007431) Mouse Recombinant Protein

• Background:

  This gene encodes a preproprotein that is proteolytically cleaved to yield a signal peptide and a proproptein that is subsequently processed to generate the active mature peptide. The encoded protein is a membrane-bound glycosylated enzyme that catalyzes the hydrolysis of phosphate esters at alkaline pH. The mature peptide maintains the ratio of inorganic phosphate to inorganic pyrophosphate required for bone mineralization. Mice that lack this enzyme show symptoms of osteomalacia, softening of the bones. In humans, mutations in this gene are associated with hypophosphatasia, an inherited metabolic bone disease in which deficiency of this enzyme inhibits bone mineralization leading to skeletal defects. Mutations in the mouse gene mirror the symptoms of human hypophosphatasia. A pseudogene of this gene is present on chromosome X. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015]

• Synonyms:

  Ak; Akp; Akp-2; Akp2; ALP; APTNAP; T; TNAP; TNSALP

• Gene ID:

  11647

• UniProt:

  P09242, Q3TQ02

• Accession Number mRNA:

  NM_007431

• Chromosomal Location:

  4 70.02 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  57.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Alpl

• Species:

  Mouse

• Protein ID:

  NP_031457

• Overview:

  Purified recombinant protein of Mouse alkaline phosphatase, liver/bone/kidney (Alpl), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/11647

• Uniprot URL:

  https://www.uniprot.org/uniprot/P09242

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_007431

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_031457