Usp2 (NM_016808) Mouse Recombinant Protein

• Background:

  Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1 (By similarity) . Isoform 1 and isoform 2 possess both ubiquitin-specific peptidase and isopeptidase activities (By similarity) . Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity (By similarity) . Has no deubiquitinase activity against p53/TP53 (By similarity) . Prevents MDM2-mediated degradation of MDM4 (By similarity) . Plays a role in the G1/S cell-cycle progression in normal and cancer cells (By similarity) . Plays a role in the regulation of myogenic differentiation of embryonic muscle cells (By similarity) . Regulates the circadian clock by modulating its intrinsic circadian rhythm and its capacity to respond to external cues (PubMed:23213472, PubMed:25238854, PubMed:26756164) . Associates with clock proteins and deubiquitinates core clock component PER1 but does not affect its overall stability (PubMed:23213472) . Regulates the nucleocytoplasmic shuttling and nuclear retention of PER1 and its repressive role on the clock transcription factors CLOCK and ARNTL/BMAL1 (PubMed:25238854) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  B930035K21Rik; Ubp41

• Gene ID:

  53376

• UniProt:

  O88623

• Accession Number mRNA:

  NM_016808

• Chromosomal Location:

  9 A5.1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  70 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Usp2

• Species:

  Mouse

• Protein ID:

  NP_058088

• Overview:

  Purified recombinant protein of Mouse ubiquitin specific peptidase 2 (Usp2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/53376

• Uniprot URL:

  https://www.uniprot.org/uniprot/O88623

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_016808

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_058088