Polk (BC052820) Mouse Recombinant Protein

• Background:

  DNA polymerase specifically involved in DNA repair. Plays an important role in translesion synthesis, where the normal high-fidelity DNA polymerases cannot proceed and DNA synthesis stalls (PubMed:12432099) . Depending on the context, it inserts the correct base, but causes frequent base transitions, transversions and frameshifts. Lacks 3'-5' proofreading exonuclease activity. Forms a Schiff base with 5'-deoxyribose phosphate at abasic sites, but does not have lyase activity (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Dinb1

• Gene ID:

  27015

• UniProt:

  Q9QUG2

• Accession Number mRNA:

  BC052820

• Chromosomal Location:

  13 D1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  82.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Polk

• Species:

  Mouse

• Overview:

  Purified recombinant protein of Mouse polymerase (DNA directed), kappa (cDNA clone MGC:60610 IMAGE:30057338), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/27015

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9QUG2

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/BC052820