Cnot3 (NM_146176) Mouse Recombinant Protein

• Background:

  Component of the CCR4-NOT complex which is one of the major cellular mRNA deadenylases and is linked to various cellular processes including bulk mRNA degradation, miRNA-mediated repression, translational repression during translational initiation and general transcription regulation. Additional complex functions may be a consequence of its influence on mRNA expression. May be involved in metabolic regulation; may be involved in recruitment of the CCR4-NOT complex to deadenylation target mRNAs involved in energy metabolism. Involved in mitotic progression and regulation of the spindle assembly checkpoint by regulating the stability of MAD1L1 mRNA. Can repress transcription and may link the CCR4-NOT complex to transcriptional regulation; the repressive function may involve histone deacetylases. Involved in the maintenance of embryonic stem (ES) cell identity; prevents their differentiation towards extraembryonic trophectoderm lineages.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  A930039N10Rik

• Gene ID:

  232791

• UniProt:

  Q8K0V4

• Accession Number mRNA:

  NM_146176

• Chromosomal Location:

  7 A1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  82.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Cnot3

• Species:

  Mouse

• Protein ID:

  NP_666288

• Overview:

  Purified recombinant protein of Mouse CCR4-NOT transcription complex, subunit 3 (Cnot3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/232791

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8K0V4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_146176

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_666288