Zw10 (NM_012039) Mouse Recombinant Protein

• Background:

  Essential component of the mitotic checkpoint, which prevents cells from prematurely exiting mitosis. Required for the assembly of the dynein-dynactin and MAD1-MAD2 complexes onto kinetochores. Its function related to the spindle assembly machinery is proposed to depend on its association in the mitotic RZZ complex. Involved in regulation of membrane traffic between the Golgi and the endoplasmic reticulum (ER) ; the function is proposed to depend on its association in the interphase NRZ complex which is believed to play a role in SNARE assembly at the ER (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  6330566F14Rik; MmZw10

• Gene ID:

  26951

• UniProt:

  O54692

• Accession Number mRNA:

  NM_012039

• Chromosomal Location:

  9 A5.3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  88.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Zw10

• Species:

  Mouse

• Protein ID:

  NP_036169

• Overview:

  Purified recombinant protein of Mouse zw10 kinetochore protein (Zw10), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/26951

• Uniprot URL:

  https://www.uniprot.org/uniprot/O54692

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_012039

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_036169