Arnt (NM_001037737) Mouse Recombinant Protein

• Background:

  Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens (By similarity) . The heterodimer binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters and functions as a transcriptional regulator of the adaptive response to hypoxia (PubMed:26245371, PubMed:27782878) . The heterodimer ARNT:AHR binds to core DNA sequence 5'-TGCGTG-3' within the dioxin response element (DRE) of target gene promoters and activates their transcription (PubMed:28602820) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  BHLHe2; D3Ertd557e; Drnt; ESTM42; Hif1b; mKIAA4051; W08714

• Gene ID:

  11863

• UniProt:

  P53762, Q3ULM2

• Accession Number mRNA:

  NM_001037737

• Chromosomal Location:

  3 40.74 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  87 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Arnt

• Species:

  Mouse

• Protein ID:

  NP_001032826

• Overview:

  Purified recombinant protein of Mouse aryl hydrocarbon receptor nuclear translocator (Arnt), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/11863

• Uniprot URL:

  https://www.uniprot.org/uniprot/P53762

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001037737

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001032826