Osbpl3 (BC086485) Mouse Recombinant Protein

• Background:

  Phosphoinositide-binding protein which associates with both cell and endoplasmic reticulum (ER) membranes. Can bind to the ER membrane protein VAPA and recruit VAPA to plasma membrane sites, thus linking these intracellular compartments. The ORP3-VAPA complex stimulates RRAS signaling which in turn attenuates integrin beta-1 (ITGB1) activation at the cell surface. With VAPA, may regulate ER morphology. Has a role in regulation of the actin cytoskeleton, cell polarity and cell adhesion. Binds to phosphoinositides with preference for PI (3,4) P2 and PI (3,4,5) P3. Also binds 25-hydroxycholesterol and cholesterol.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  ORP3, OSBP3, A530055M08

• Gene ID:

  71720

• UniProt:

  Q9DBS9

• Accession Number mRNA:

  BC086485

• Chromosomal Location:

  6 B2.3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  118.5 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Osbpl3

• Species:

  Mouse

• Overview:

  Purified recombinant protein of Mouse oxysterol binding protein-like 3 (cDNA clone MGC:102333 IMAGE:5344992), complete cds, with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/71720

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9DBS9

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/BC086485