Actn4 (NM_021895) Mouse Recombinant Protein

• Background:

  F-actin cross-linking protein which is thought to anchor actin to a variety of intracellular structures. This is a bundling protein. Probably involved in vesicular trafficking via its association with the CART complex. The CART complex is necessary for efficient transferrin receptor recycling but not for EGFR degradation (By similarity) . Involved in tight junction assembly in epithelial cells probably through interaction with MICALL2. Links MICALL2 to the actin cytoskeleton and recruits it to the tight junctions (PubMed:18332111) . May also function as a transcriptional coactivator, stimulating transcription mediated by the nuclear hormone receptors PPARG and RARA (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  C77391

• Gene ID:

  60595

• UniProt:

  P57780, Q3ULT2

• Accession Number mRNA:

  NM_021895

• Chromosomal Location:

  7 B1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  105.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Actn4

• Species:

  Mouse

• Protein ID:

  NP_068695

• Overview:

  Purified recombinant protein of Mouse actinin alpha 4 (Actn4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/60595

• Uniprot URL:

  https://www.uniprot.org/uniprot/P57780

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_021895

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_068695