Epha1 (NM_023580) Mouse Recombinant Protein

• Background:

  Receptor tyrosine kinase which binds promiscuously membrane-bound ephrin-A family ligands residing on adjacent cells, leading to contact-dependent bidirectional signaling into neighboring cells. The signaling pathway downstream of the receptor is referred to as forward signaling while the signaling pathway downstream of the ephrin ligand is referred to as reverse signaling. Binds with a low affinity EFNA3 and EFNA4 and with a high affinity to EFNA1 which most probably constitutes its cognate/functional ligand. Upon activation by EFNA1 induces cell attachment to the extracellular matrix inhibiting cell spreading and motility through regulation of ILK and downstream RHOA and RAC. Plays also a role in angiogenesis and regulates cell proliferation. May play a role in apoptosis.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  5730453L17Rik; AL033318; Eph; Esk

• Gene ID:

  13835

• UniProt:

  Q60750

• Accession Number mRNA:

  NM_023580

• Chromosomal Location:

  6 B2.1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  108.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Epha1

• Species:

  Mouse

• Protein ID:

  NP_076069

• Overview:

  Purified recombinant protein of Mouse Eph receptor A1 (Epha1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/13835

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q60750

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_023580

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_076069