Spag5 (NM_017407) Mouse Recombinant Protein

• Background:

  Essential component of the mitotic spindle required for normal chromosome segregation and progression into anaphase. Required for chromosome alignment, normal timing of sister chromatid segregation, and maintenance of spindle pole architecture. In complex with SKAP, promotes stable microtubule-kinetochore attachments. May contribute to the regulation of separase activity. May regulate AURKA localization to mitotic spindle, but not to centrosomes and CCNB1 localization to both mitotic spindle and centrosomes. Involved in centriole duplication. Required for CDK5RAP22, CEP152, WDR62 and CEP63 centrosomal localization and promotes the centrosomal localization of CDK2. In non-mitotic cells, upon stress induction, inhibits mammalian target of rapamycin complex 1 (mTORC1) association and recruits the mTORC1 component RPTOR to stress granules (SGs), thereby preventing mTORC1 hyperactivation-induced apoptosis. May enhance GSK3B-mediated phosphorylation of other substrates, such as MAPT/TAU (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI874642; D11Bhm180e; Deepest; MAP126; Mastrin; S17

• Gene ID:

  54141

• UniProt:

  Q7TME2

• Accession Number mRNA:

  NM_017407

• Chromosomal Location:

  11 46.74 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  130.4 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Spag5

• Species:

  Mouse

• Protein ID:

  NP_059103

• Overview:

  Purified recombinant protein of Mouse sperm associated antigen 5 (Spag5), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/54141

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q7TME2

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_017407

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_059103