Otud7b (NM_001025613) Mouse Recombinant Protein

• Background:

  Negative regulator of the non-canonical NF-kappa-B pathway that acts by mediating deubiquitination of TRAF3, an inhibitor of the NF-kappa-B pathway, thereby acting as a negative regulator of B-cell responses. In response to non-canonical NF-kappa-B stimuli, deubiquitinates 'Lys-48'-linked polyubiquitin chains of TRAF3, preventing TRAF3 proteolysis and over-activation of non-canonical NF-kappa-B (PubMed:23334419) . Negatively regulates mucosal immunity against infections (PubMed:23334419) . Deubiquitinates ZAP70, and thereby regulates T cell receptor (TCR) signaling that leads to the activation of NF-kappa-B (PubMed:26903241) . Plays a role in T cell homeostasis and is required for normal T cell responses, including production of IFNG and IL2 (PubMed:26903241) . Mediates deubiquitination of EGFR (By similarity) . Has deubiquitinating activity toward 'Lys-11', 'Lys-48' and 'Lys-63'-linked polyubiquitin chains. Has a much higher catalytic rate with 'Lys-11'-linked polyubiquitin chains (in vitro) ; however the physiological significance of these data are unsure. Hydrolyzes both linear and branched forms of polyubiquitin (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  2900060B22Rik; 4930463P07Rik; AI462125; CEZANNE; Trabid2; Za20d1

• Gene ID:

  229603

• UniProt:

  B2RUR8

• Accession Number mRNA:

  NM_001025613

• Chromosomal Location:

  3 F2.1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  92 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Otud7b

• Species:

  Mouse

• Protein ID:

  NP_001020784

• Overview:

  Purified recombinant protein of Mouse OTU domain containing 7B (Otud7b), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/229603

• Uniprot URL:

  https://www.uniprot.org/uniprot/B2RUR8

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001025613

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001020784