Osbp (NM_001033174) Mouse Recombinant Protein

• Background:

  Lipid transporter involved in lipid countertransport between the Golgi complex and membranes of the endoplasmic reticulum: specifically exchanges sterol with phosphatidylinositol 4-phosphate (PI4P), delivering sterol to the Golgi in exchange for PI4P, which is degraded by the SAC1/SACM1L phosphatase in the endoplasmic reticulum. Binds cholesterol and a range of oxysterols including 25-hydroxycholesterol. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylates ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  1110018F06Rik; AW559088; mKIAA4220

• Gene ID:

  76303

• UniProt:

  Q3B7Z2

• Accession Number mRNA:

  NM_001033174

• Chromosomal Location:

  19 8.58 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  89.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Osbp

• Species:

  Mouse

• Protein ID:

  NP_001028346

• Overview:

  Purified recombinant protein of Mouse oxysterol binding protein (Osbp), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/76303

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q3B7Z2

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001033174

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001028346