Kars (NM_053092) Mouse Recombinant Protein

• Background:

  Catalyzes the specific attachment of an amino acid to its cognate tRNA in a 2 step reaction: the amino acid (AA) is first activated by ATP to form AA-AMP and then transferred to the acceptor end of the tRNA. When secreted, acts as a signaling molecule that induces immune response through the activation of monocyte/macrophages. Catalyzes the synthesis of the signaling molecule diadenosine tetraphosphate (Ap4A), and thereby mediates disruption of the complex between HINT1 and MITF and the concomitant activation of MITF transcriptional activity.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AA589550; AL024334; AL033315; AL033367; D8Ertd698e; D8Wsu108e; LysRS; mKIAA0070

• Gene ID:

  85305

• UniProt:

  Q99MN1, Q3TIV6

• Accession Number mRNA:

  NM_053092

• Chromosomal Location:

  8 58.27 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  67.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Kars

• Species:

  Mouse

• Protein ID:

  NP_444322

• Overview:

  Purified recombinant protein of Mouse lysyl-tRNA synthetase (Kars), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/85305

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q99MN1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_053092

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_444322