Cenpt (NM_177150) Mouse Recombinant Protein

• Background:

  Component of the CENPA-NAC (nucleosome-associated) complex, a complex that plays a central role in assembly of kinetochore proteins, mitotic progression and chromosome segregation. The CENPA-NAC complex recruits the CENPA-CAD (nucleosome distal) complex and may be involved in incorporation of newly synthesized CENPA into centromeres. Part of a nucleosome-associated complex that binds specifically to histone H3-containing nucleosomes at the centromere, as opposed to nucleosomes containing CENPA. Component of the heterotetrameric CENP-T-W-S-X complex that binds and supercoils DNA, and plays an important role in kinetochore assembly. CENPT has a fundamental role in kinetochore assembly and function. It is one of the inner kinetochore proteins, with most further proteins binding downstream. Required for normal chromosome organization and normal progress through mitosis.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  G630055P03Rik

• Gene ID:

  320394

• UniProt:

  Q3TJM4

• Accession Number mRNA:

  NM_177150

• Chromosomal Location:

  8 D3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  56.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Cenpt

• Species:

  Mouse

• Protein ID:

  NP_796124

• Overview:

  Purified recombinant protein of Mouse centromere protein T (Cenpt), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/320394

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q3TJM4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_177150

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_796124