Plcd4 (NM_148937) Mouse Recombinant Protein

• Background:

  Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) . DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca (2+) from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca (2+) mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  4921507K24Rik

• Gene ID:

  18802

• UniProt:

  Q8K3R3

• Accession Number mRNA:

  NM_148937

• Chromosomal Location:

  1 38.54 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  92.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Plcd4

• Species:

  Mouse

• Protein ID:

  NP_683739

• Overview:

  Purified recombinant protein of Mouse phospholipase C, delta 4 (Plcd4), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/18802

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8K3R3

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_148937

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_683739