Exd1 (NM_172857) Mouse Recombinant Protein

• Background:

  RNA-binding component of the PET complex, a multiprotein complex required for the processing of piRNAs during spermatogenesis. The piRNA metabolic process mediates the repression of transposable elements during meiosis by forming complexes composed of piRNAs and Piwi proteins and governs the methylation and subsequent repression of transposable elements, preventing their mobilization, which is essential for the germline integrity (PubMed:26669262) . The PET complex is required during the secondary piRNAs metabolic process for the PIWIL2 slicing-triggered loading of PIWIL4 piRNAs. In the PET complex, EXD1 probably acts as an RNA adapter. EXD1 is an inactive exonuclease (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  4932702D22Rik; Exdl1; mExd1

• Gene ID:

  241624

• UniProt:

  Q8CDF7

• Accession Number mRNA:

  NM_172857

• Chromosomal Location:

  2 E5

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  64 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Exd1

• Species:

  Mouse

• Protein ID:

  NP_766445

• Overview:

  Purified recombinant protein of Mouse exonuclease 3'-5' domain containing 1 (Exd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/241624

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8CDF7

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_172857

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_766445