Rnf7 (NM_011279) Mouse Recombinant Protein

• Background:

  Probable component of the SCF (SKP1-CUL1-F-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins involved in cell cycle progression, signal transduction and transcription (By similarity) . CRLs complexes and ARIH1 collaborate in tandem to mediate ubiquitination of target proteins, ARIH1 mediating addition of the first ubiquitin on CRLs targets (By similarity) . Through the RING-type zinc finger, seems to recruit the E2 ubiquitination enzyme to the complex and brings it into close proximity to the substrate. Promotes the neddylation of CUL5 via its interaction with UBE2F. May play a role in protecting cells from apoptosis induced by redox agents (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Rbx2; SAG

• Gene ID:

  19823

• UniProt:

  Q9WTZ1

• Accession Number mRNA:

  NM_011279

• Chromosomal Location:

  9 E3.3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  12.7 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Rnf7

• Species:

  Mouse

• Protein ID:

  NP_035409

• Overview:

  Purified recombinant protein of Mouse ring finger protein 7 (Rnf7), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/19823

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9WTZ1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_011279

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_035409