F13a1 (NM_001166391) Mouse Recombinant Protein

• Background:

  This gene encodes subunit A of the coagulation factor XIII that catalyzes the final step of the blood coagulation pathway. The encoded protein associates with subunit B to form a heterotetrameric proenzyme that undergoes thrombin-mediated proteolysis to generate active factor XIIIa. The transglutaminase activity of factor XIIIa is required for the calcium-dependent crosslinking of fibrin, leading to the formation of a clot. Mice lacking the encoded protein display impaired reproduction and reduced survival due to bleeding episodes, hematothorax, hematoperitoneum and subcutaneous hemorrhage. Additionally, mice lacking the encoded protein exhibit impaired wound healing and inadequate healing of myocardial infarction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

• Synonyms:

  1200014I03Rik; AI462306; F13a

• Gene ID:

  74145

• UniProt:

  Q8BH61, Q3V3W7

• Accession Number mRNA:

  NM_001166391

• Chromosomal Location:

  13 A3.3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  83.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  F13a1

• Species:

  Mouse

• Protein ID:

  NP_001159863

• Overview:

  Purified recombinant protein of Mouse coagulation factor XIII, A1 subunit (F13a1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/74145

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q8BH61

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001166391

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001159863