Flt3 (NM_010229) Mouse Recombinant Protein

• Background:

  Tyrosine-protein kinase that acts as cell-surface receptor for the cytokine FLT3LG and regulates differentiation, proliferation and survival of hematopoietic progenitor cells and of dendritic cells. Promotes phosphorylation of SHC1 and AKT1, and activation of the downstream effector MTOR. Promotes activation of RAS signaling and phosphorylation of downstream kinases, including MAPK1/ERK2 and/or MAPK3/ERK1. Promotes phosphorylation of FES, FER, PTPN6/SHP, PTPN11/SHP-2, PLCG1, and STAT5A and/or STAT5B. Activation of wild-type FLT3 causes only marginal activation of STAT5A or STAT5B. Mutations that cause constitutive kinase activity promote cell proliferation and resistance to apoptosis via the activation of multiple signaling pathways.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  B230315G04; CD135; Flk-2; Flk2; Flt-3; Ly72; wmfl

• Gene ID:

  14255

• UniProt:

  Q00342, Q3UEW6, Q3TQF1

• Accession Number mRNA:

  NM_010229

• Chromosomal Location:

  5 G3

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  113.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Flt3

• Species:

  Mouse

• Protein ID:

  NP_034359

• Overview:

  Purified recombinant protein of Mouse FMS-like tyrosine kinase 3 (Flt3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/14255

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q00342

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_010229

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_034359