Hey2 (NM_013904) Mouse Recombinant Protein

• Background:

  Transcriptional repressor which functions as a downstream effector of Notch signaling in cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. May be required in conjunction with HEY1 to specify arterial cell fate or identity. Promotes maintenance of neuronal precursor cells and glial versus neuronal fate specification. Binds preferentially to the canonical E box sequence 5'-CACGTG-3'. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6 and by the neuronal bHLH factors ASCL1/MASH1 and NEUROD4/MATH3.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  BHLHb32; CHF1; Herp1; hesr2; Hrt2

• Gene ID:

  15214

• UniProt:

  Q9QUS4, Q0VGJ1

• Accession Number mRNA:

  NM_013904

• Chromosomal Location:

  10 A4

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  36.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Hey2

• Species:

  Mouse

• Protein ID:

  NP_038932

• Overview:

  Purified recombinant protein of Mouse hairy/enhancer-of-split related with YRPW motif 2 (Hey2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/15214

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9QUS4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_013904

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_038932