Erap1 (NM_030711) Mouse Recombinant Protein

• Background:

  Aminopeptidase that plays a central role in peptide trimming, a step required for the generation of most HLA class I-binding peptides. Peptide trimming is essential to customize longer precursor peptides to fit them to the correct length required for presentation on MHC class I molecules. Strongly prefers substrates 9-16 residues long. Rapidly degrades 13-mer to a 9-mer and then stops. Preferentially hydrolyzes the residue Leu and peptides with a hydrophobic C-terminus, while it has weak activity toward peptides with charged C-terminus. May play a role in the inactivation of peptide hormones. May be involved in the regulation of blood pressure through the inactivation of angiotensin II and/or the generation of bradykinin in the kidney (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Arts1; ERAAP; PILSA; PILSAP

• Gene ID:

  80898

• UniProt:

  Q9EQH2

• Accession Number mRNA:

  NM_030711

• Chromosomal Location:

  13 C1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  107 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Erap1

• Species:

  Mouse

• Protein ID:

  NP_109636

• Overview:

  Purified recombinant protein of Mouse endoplasmic reticulum aminopeptidase 1 (Erap1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/80898

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9EQH2

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_030711

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_109636