Aifm1 (NM_012019) Mouse Recombinant Protein

• Background:

  Functions both as NADH oxidoreductase and as regulator of apoptosis. In response to apoptotic stimuli, it is released from the mitochondrion intermembrane space into the cytosol and to the nucleus, where it functions as a proapoptotic factor in a caspase-independent pathway. In contrast, functions as an antiapoptotic factor in normal mitochondria via its NADH oxidoreductase activity. The soluble form (AIFsol) found in the nucleus induces 'parthanatos' i.e. caspase-independent fragmentation of chromosomal DNA. Interacts with EIF3G, and thereby inhibits the EIF3 machinery and protein synthesis, and activates casapse-7 to amplify apoptosis. Plays a critical role in caspase-independent, pyknotic cell death in hydrogen peroxide-exposed cells. Binds to DNA in a sequence-independent manner (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AIF; AIFsh2; Hq; Pdcd8

• Gene ID:

  26926

• UniProt:

  Q9Z0X1

• Accession Number mRNA:

  NM_012019

• Chromosomal Location:

  X 25.68 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  67.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Aifm1

• Species:

  Mouse

• Protein ID:

  NP_036149

• Overview:

  Purified recombinant protein of Mouse apoptosis-inducing factor, mitochondrion-associated 1 (Aifm1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/26926

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9Z0X1

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_012019

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_036149