Pou3f2 (NM_008899) Mouse Recombinant Protein

• Background:

  Transcription factor that plays a key role in neuronal differentiation (PubMed:24243019) . Binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, 2, or 3 nucleotides (By similarity) . The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220) . Acts downstream of ASCL1, accessing chromatin that has been opened by ASCL1, and promotes transcription of neuronal genes (PubMed:24243019) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  9430075J19Rik; A230098E07Rik; Brn-2; Brn2; oct-7; OTF-7; Otf7

• Gene ID:

  18992

• UniProt:

  P31360

• Accession Number mRNA:

  NM_008899

• Chromosomal Location:

  4 9.73 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  47.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Pou3f2

• Species:

  Mouse

• Protein ID:

  NP_032925

• Overview:

  Purified recombinant protein of Mouse POU domain, class 3, transcription factor 2 (Pou3f2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/18992

• Uniprot URL:

  https://www.uniprot.org/uniprot/P31360

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008899

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032925