Socs3 (NM_007707) Mouse Recombinant Protein

• Background:

  SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo. Probable substrate-recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins (By similarity) . Seems to recognize IL6ST.[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Cis3; Cish3; EF-10; Ef10; SSI-3; Ssi3

• Gene ID:

  12702

• UniProt:

  O35718

• Accession Number mRNA:

  NM_007707

• Chromosomal Location:

  11 E2

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  25.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Socs3

• Species:

  Mouse

• Protein ID:

  NP_031733

• Overview:

  Purified recombinant protein of Mouse suppressor of cytokine signaling 3 (Socs3), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/12702

• Uniprot URL:

  https://www.uniprot.org/uniprot/O35718

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_007707

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_031733