Rnf2 (NM_011277) Mouse Recombinant Protein

• Background:

  E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A (H2AK119Ub), thereby playing a central role in histone code and gene regulation (PubMed:15525528, PubMed:22325148, PubMed:28596365) . H2AK119Ub gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals (PubMed:15525528, PubMed:28596365) . May be involved in the initiation of both imprinted and random X inactivation (PubMed:15525528) . Essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development (PubMed:22325148, PubMed:16710298) . PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility (PubMed:15525528, PubMed:22325148, PubMed:16710298) . E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4 (PubMed:16710298) . Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity (PubMed:15525528, PubMed:16710298) . Plays a role in the transcriptional repression of genes that are required for pluripotency in embryonic stem cells, thereby contributing to differentiation of the ectodermal and endodermal germ layers (PubMed:22226355) . Association with the chromosomal DNA is cell-cycle dependent. In resting B- and T-lymphocytes, interaction with AURKB leads to block its activity, thereby maintaining transcription in resting lymphocytes (PubMed:24034696) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI326319; AI450156; AU019207; dinG; Ring1B

• Gene ID:

  19821

• UniProt:

  Q9CQJ4

• Accession Number mRNA:

  NM_011277

• Chromosomal Location:

  1 G1

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  38.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Rnf2

• Species:

  Mouse

• Protein ID:

  NP_035407

• Overview:

  Purified recombinant protein of Mouse ring finger protein 2 (Rnf2), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/19821

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9CQJ4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_011277

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_035407