Zap70 (NM_009539) Mouse Recombinant Protein

• Background:

  This gene encodes a member of the protein tyrosine kinase family. The encoded protein is essential for development of T lymphocytes and thymocytes, and functions in the initial step of T lymphocyte receptor-mediated signal transduction. A mutation in this gene causes chronic autoimmune arthritis, similar to rheumatoid arthritis in humans. Mice lacking this gene are deficient in alpha-beta T lymphocytes in the thymus. In humans, mutations in this gene cause selective T-cell defect, a severe combined immunodeficiency disease characterized by a selective absence of CD8-positive T lymphocytes. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]

• Synonyms:

  Mrtle; mur; S; Srk; ZAP-; ZAP-70

• Gene ID:

  22637

• UniProt:

  P43404

• Accession Number mRNA:

  NM_009539

• Chromosomal Location:

  1 15.41 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  70.6 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Zap70

• Species:

  Mouse

• Protein ID:

  NP_033565

• Overview:

  Purified recombinant protein of Mouse zeta-chain (TCR) associated protein kinase (Zap70), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/22637

• Uniprot URL:

  https://www.uniprot.org/uniprot/P43404

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_009539

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_033565