Ccnd1 (NM_007631) Mouse Recombinant Protein

• Background:

  Regulatory component of the cyclin D1-CDK4 (DC) complex that phosphorylates and inhibits members of the retinoblastoma (RB) protein family including RB1 and regulates the cell-cycle during G (1) /S transition. Phosphorylation of RB1 allows dissociation of the transcription factor E2F from the RB/E2F complex and the subsequent transcription of E2F target genes which are responsible for the progression through the G (1) phase. Hypophosphorylates RB1 in early G (1) phase. Cyclin D-CDK4 complexes are major integrators of various mitogenenic and antimitogenic signals. Also substrate for SMAD3, phosphorylating SMAD3 in a cell-cycle-dependent manner and repressing its transcriptional activity. Component of the ternary complex, cyclin D1/CDK4/CDKN1B, required for nuclear translocation and activity of the cyclin D-CDK4 complex. Exhibits transcriptional corepressor activity with INSM1 on the NEUROD1 and INS promoters in a cell cycle-independent manner (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  AI327039; bcl-1; cD1; CycD1; Cyl-1; PRAD1

• Gene ID:

  12443

• UniProt:

  P25322, Q790L7

• Accession Number mRNA:

  NM_007631

• Chromosomal Location:

  7 88.92 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  33.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Ccnd1

• Species:

  Mouse

• Protein ID:

  NP_031657

• Overview:

  Purified recombinant protein of Mouse cyclin D1 (Ccnd1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/12443

• Uniprot URL:

  https://www.uniprot.org/uniprot/P25322

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_007631

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_031657