Trim33 (NM_053170) Mouse Recombinant Protein

• Background:

  Acts as an E3 ubiquitin-protein ligase. Promotes SMAD4 ubiquitination, nuclear exclusion and degradation via the ubiquitin proteasome pathway (By similarity) . May act as a transcriptional repressor (By similarity) . Inhibits the transcriptional response to TGF-beta/BMP signaling cascade (By similarity) . Plays a role in the control of cell proliferation (By similarity) . Its association with SMAD2 and SMAD3 stimulates erythroid differentiation of hematopoietic stem/progenitor. Monoubiquitinates SMAD4 and acts as an inhibitor of SMAD4-dependent TGF-beta/BMP signaling cascade (Monoubiquitination of SMAD4 hampers its ability to form a stable complex with activated SMAD2/3 resulting in inhibition of TGF-beta/BMP signaling cascade) (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  8030451N04Rik; AI413936; Ecto; Tif1g

• Gene ID:

  94093

• UniProt:

  Q99PP7, E9QP19

• Accession Number mRNA:

  NM_053170

• Chromosomal Location:

  3 F2.2

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  124.1 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Trim33

• Species:

  Mouse

• Protein ID:

  NP_444400

• Overview:

  Purified recombinant protein of Mouse tripartite motif-containing 33 (Trim33), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/94093

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q99PP7

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_053170

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_444400