Tjp1 (NM_009386) Mouse Recombinant Protein

• Background:

  Tjp1, TjpP2, and Tjp3 are closely related scaffolding proteins that link tight junction (TJ) transmembrane proteins such as claudins, junctional adhesion molecules, and occludin to the actin cytoskeleton (By similarity) . The tight junction acts to limit movement of substances through the paracellular space and as a boundary between the compositionally distinct apical and basolateral plasma membrane domains of epithelial and endothelial cells. Necessary for lumenogenesis, and particularly efficient epithelial polarization and barrier formation (By similarity) . Plays a role in the regulation of cell migration by targeting Cdc42bpb to the leading edge of migrating cells (By similarity) . Plays an important role in podosome formation and associated function, thus regulating cell adhesion and matrix remodeling (By similarity) . With Tjp2 and TJjp3, participates to the junctional retention and stability of the transcription factor Dbpa, but is not involved in its shuttling to the nucleus (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  ZO1

• Gene ID:

  21872

• UniProt:

  P39447

• Accession Number mRNA:

  NM_009386

• Chromosomal Location:

  7 35.02 cM

• Expression System:

  HEK293T

• Tag:

  C-MYC/DDK

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

• Molecular Weight:

  195.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Tjp1

• Species:

  Mouse

• Protein ID:

  NP_033412

• Overview:

  Purified recombinant protein of Mouse tight junction protein 1 (Tjp1), with C-terminal MYC/DDK tag, expressed in HEK293T cells, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/21872

• Uniprot URL:

  https://www.uniprot.org/uniprot/P39447

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_009386

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_033412