Pdcd1 (NM_008798) Mouse Recombinant Protein

• Background:

  Inhibitory receptor on antigen activated T-cells that plays a critical role in induction and maintenance of immune tolerance to self (PubMed:10485649, PubMed:11698646, PubMed:11209085, PubMed:21300912) . Delivers inhibitory signals upon binding to ligands, such as CD274/PDCD1L1 and CD273/PDCD1LG2 (PubMed:11015443, PubMed:11224527, PubMed:22641383, PubMed:18287011, PubMed:18641123) . Following T-cell receptor (TCR) engagement, PDCD1 associates with CD3-TCR in the immunological synapse and directly inhibits T-cell activation (PubMed:22641383) . Suppresses T-cell activation through the recruitment of PTPN11/SHP-2: following ligand-binding, PDCD1 is phosphorylated within the ITSM motif, leading to the recruitment of the protein tyrosine phosphatase PTPN11/SHP-2 that mediates dephosphorylation of key TCR proximal signaling molecules, such as ZAP70, PRKCQ/PKCtheta and CD247/CD3zeta (PubMed:11698646, PubMed:22641383) . The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity) .[UniProtKB/Swiss-Prot Function]

• Synonyms:

  Ly101; PD-1; Pdc1

• Gene ID:

  18566

• UniProt:

  Q02242, Q544F3

• Accession Number mRNA:

  NM_008798

• Chromosomal Location:

  1 D

• Expression System:

  HEK293

• Tag:

  C-DDK/His

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  PBS, pH7.4, 10% glycerol

• Molecular Weight:

  19 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Pdcd1

• Species:

  Mouse

• Protein ID:

  NP_032824

• Overview:

  Purified recombinant protein of mouse programmed cell death 1 (PD-1 / PDCD1), with C-terminal DDK/His tag, expressed in human cells, 20 µg

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/18566

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q02242

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008798

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032824