Thymidylate Synthase (TYMS) (NM_001071) Human Recombinant Protein

• Background:

  Thymidylate synthase catalyzes the methylation of deoxyuridylate to deoxythymidylate using, 10-methylenetetrahydrofolate (methylene-THF) as a cofactor. This function maintains the dTMP (thymidine-5-prime monophosphate) pool critical for DNA replication and repair. The enzyme has been of interest as a target for cancer chemotherapeutic agents. It is considered to be the primary site of action for 5-fluorouracil, 5-fluoro-2-prime-deoxyuridine, and some folate analogs. Expression of this gene and that of a naturally occurring antisense transcript, mitochondrial enolase superfamily member 1 (GeneID:55556), vary inversely when cell-growth progresses from late-log to plateau phase. Polymorphisms in this gene may be associated with etiology of neoplasia, including breast cancer, and response to chemotherapy. [provided by RefSeq, Aug 2017]

• Synonyms:

  HST422; TMS; TS

• Gene ID:

  7298

• UniProt:

  P04818, Q53Y97

• Accession Number mRNA:

  NM_001071

• Chromosomal Location:

  18p11.32

• Expression System:

  Sf9

• Tag:

  N-His

• Related Pathways:

  Metabolic pathways, One carbon pool by folate, Pyrimidine metabolism

• Field of Research:

  Metabolic pathways, One carbon pool by folate, Pyrimidine metabolism

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris-HCl pH8.0, 150mM NaCl, 10% glycerol

• Function:

  Druggable Genome

• Molecular Weight:

  36 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  TYMS

• Species:

  Human

• Protein ID:

  NP_001062

• Overview:

  Recombinant protein of human thymidylate synthetase (TYMS), full length, with N-terminal polyhistidine tag, expressed in sf9 cells.

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/7298

• Uniprot URL:

  https://www.uniprot.org/uniprot/P04818

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_001071

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_001062