Cyclin D1 (CCND1) (NM_053056) Human Recombinant Protein

• Background:

  The protein encoded by this gene belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of human cancers. [provided by RefSeq, Dec 2019]

• Synonyms:

  BCL1; D11S287E; PRAD1; U21B31

• Gene ID:

  595

• UniProt:

  P24385, Q6FI00

• Accession Number mRNA:

  NM_053056

• Chromosomal Location:

  11q13.3

• Expression System:

  Sf9

• Tag:

  C-DDK

• Related Pathways:

  Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Focal adhesion, Glioma, Jak-STAT signaling pathway, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Viral myocarditis, Wnt signaling pathway

• Field of Research:

  Acute myeloid leukemia, Bladder cancer, Cell cycle, Chronic myeloid leukemia, Colorectal cancer, Endometrial cancer, Focal adhesion, Glioma, Jak-STAT signaling pathway, Melanoma, Non-small cell lung cancer, p53 signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, Small cell lung cancer, Thyroid cancer, Viral myocarditis, Wnt signaling pathway

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris-HCl pH8.0, 150mM NaCl, 10%glycerol

• Function:

  Druggable Genome, Stem cell - Pluripotency, Stem cell relevant signaling - DSL/Notch pathway, Stem cell relevant signaling - JAK/STAT signaling pathway, Stem cell relevant signaling - Wnt Signaling pathway

• Molecular Weight:

  34 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CCND1

• Species:

  Human

• Protein ID:

  NP_444284

• Overview:

  Recombinant protein of human cyclin D1 (CCND1), full length, with C-terminal flag tag, expressed in sf9 cells

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/595

• Uniprot URL:

  https://www.uniprot.org/uniprot/P24385

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_053056

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_444284