FLT3 (NM_004119) Human Recombinant Protein

• Background:

  This gene encodes a class III receptor tyrosine kinase that regulates hematopoiesis. This receptor is activated by binding of the fms-related tyrosine kinase 3 ligand to the extracellular domain, which induces homodimer formation in the plasma membrane leading to autophosphorylation of the receptor. The activated receptor kinase subsequently phosphorylates and activates multiple cytoplasmic effector molecules in pathways involved in apoptosis, proliferation, and differentiation of hematopoietic cells in bone marrow. Mutations that result in the constitutive activation of this receptor result in acute myeloid leukemia and acute lymphoblastic leukemia. [provided by RefSeq, Jan 2015]

• Synonyms:

  CD135; FLK-2; FLK2; STK1

• Gene ID:

  2322

• UniProt:

  P36888

• Accession Number mRNA:

  NM_004119

• Chromosomal Location:

  13q12.2

• Expression System:

  Sf9

• Tag:

  C-DDK

• Related Pathways:

  Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer

• Field of Research:

  Acute myeloid leukemia, Cytokine-cytokine receptor interaction, Hematopoietic cell lineage, Pathways in cancer

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  50mM Tris-HCl, pH8.0, 100mM glycine, 10% glycerol

• Function:

  Druggable Genome, ES Cell Differentiation/IPS, Protein Kinase, Transmembrane

• Molecular Weight:

  58.3 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FLT3

• Species:

  Human

• Protein ID:

  NP_004110

• Overview:

  Recombinant protein of human fms-related tyrosine kinase 3 (FLT3), residues 27-543aa, with C-terminal DDK tag, expressed in sf9, 20ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2322

• Uniprot URL:

  https://www.uniprot.org/uniprot/P36888

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_004119

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_004110