UBE2E3 (NM_006357) Human Recombinant Protein

• Background:

  The modification of proteins with ubiquitin is an important cellular mechanism for targeting abnormal or short-lived proteins for degradation. Ubiquitination involves at least three classes of enzymes: ubiquitin-activating enzymes, or E1s, ubiquitin-conjugating enzymes, or E2s, and ubiquitin-protein ligases, or E3s. This gene encodes a member of the E2 ubiquitin-conjugating enzyme family. The encoded protein shares 100% sequence identity with the mouse and rat counterparts, which indicates that this enzyme is highly conserved in eukaryotes. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jun 2013]

• Synonyms:

  UBCH9; UbcM2

• Gene ID:

  10477

• UniProt:

  Q969T4

• Accession Number mRNA:

  NM_006357

• Chromosomal Location:

  2q31.3

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Ubiquitin mediated proteolysis

• Field of Research:

  Ubiquitin mediated proteolysis

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol

• Molecular Weight:

  22.8 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  UBE2E3

• Species:

  Human

• Protein ID:

  NP_006348

• Overview:

  Recombinant protein of human ubiquitin-conjugating enzyme E2E 3 (UBE2E3), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/10477

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q969T4

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_006357

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_006348