PPT1 (NM_000310) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a small glycoprotein involved in the catabolism of lipid-modified proteins during lysosomal degradation. The encoded enzyme removes thioester-linked fatty acyl groups such as palmitate from cysteine residues. Defects in this gene are a cause of infantile neuronal ceroid lipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis 4 (CLN4) . Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq, Dec 2008]

• Synonyms:

  CLN1; INCL; PPT

• Gene ID:

  5538

• UniProt:

  P50897

• Accession Number mRNA:

  NM_000310

• Chromosomal Location:

  1p34.2

• Expression System:

  E. coli

• Tag:

  N-His

• Related Pathways:

  Fatty acid elongation in mitochondria, Lysosome, Metabolic pathways

• Field of Research:

  Fatty acid elongation in mitochondria, Lysosome, Metabolic pathways

• Concentration:

  >50 ug/mL as determined by microplate BCA method

• Purity:

  > 80% as determined by SDS-PAGE and Coomassie blue staining

• Form:

  Liquid

• Buffer:

  25mM Tris, pH8.0, 150 mM NaCl, 10% glycerol, 1 % Sarkosyl

• Function:

  Druggable Genome

• Molecular Weight:

  34.2 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  PPT1

• Species:

  Human

• Protein ID:

  NP_000301

• Overview:

  Recombinant protein of human palmitoyl-protein thioesterase 1 (PPT1), transcript variant 1, full length, with N-terminal HIS tag, expressed in E.Coli, 50ug

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/5538

• Uniprot URL:

  https://www.uniprot.org/uniprot/P50897

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000310

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000301